A fan has been arrested and an FA investigation launched after Wycombe Wanderers goalkeeper Ryan Allsop reported being subject to alleged homophobic chanting.
Allsop made the complaint to the referee John Busby at half-time during the Tranmere-Wycombe game and Merseyside Police were alerted.
Police made one arrest and are currently investigating two other individuals.
Tranmere promised a full investigation into the claims and have said any fan found guilty of wrong doing will be punished. The FA have now opened an investigation.
After the match, Wycombe boss Gareth Ainsworth told Sky Sports: “Whether it’s racist or homophobic, whatever it is, we have to stamp this out.
“I have to give credit to Ryan Allsop for been brave enough to stand up and say I’m reporting this, and that’s what we and I’ll stand by him.”
Tranmere Rovers boss Micky Mellon said: “Obviously it is being dealt with properly. At the moment, we feel everything is being done that is necessary.
“It will play out the way people have to deal with it and I am sure they will deal with it because Tranmere is a good football club.”
Tranmere lost the match 2-0 to the league leaders and now hover to just above the relegation zone but the match will be overshadowed by the fans actions.